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Item #:
15794720
EDVOTEK SYBR Safe DNA Stain, 10,000X Concentrate, Green
78% of respondents would recommend this to a friend
Item #:
15794720
MWK 260035
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SYBR Safe is a safer alternative to Ethidium Bromide and gives more sensitive results.
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What Stands Out
High Sensitivity
Provides excellent detection for DNA in assays, significantly improving signal visibility and accuracy in gel electrophoresis compared to traditional stains.
Non-toxic Formula
Unlike other DNA stains, SYBR Safe is designed to be safer for users, reducing health risks associated with laboratory work while maintaining high performance.
Concentrated Solution
10,000X concentration allows for economical use, enabling multiple applications with minimal quantities, making it ideal for labs with budget constraints.
Product Details
| Package Weight | 1 Pound |
Who Should Buy?
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Molecular Biologists
Ideal for molecular biologists conducting DNA analysis, particularly in gel electrophoresis applications requiring sensitive staining.
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Research Labs
Perfect for research laboratories needing a reliable and safe DNA stain for various experimental setups.
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Education Institutions
Beneficial for schools and universities conducting biology experiments, ensuring safe handling and clear visual results.
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Non-Scientific Users
Not suitable for casual users without a scientific background, as its applications require specific technical knowledge.
Product Description
EDVOTEK SYBR Safe DNA Stain, 10,000X Concentrate, Green
Have any Query? Chat with us
Customer Questions & Answers
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Question:
What is EDVOTEK SYBR Safe DNA Stain used for?
Answer: EDVOTEK SYBR Safe DNA Stain is utilized in molecular biology applications for visualizing DNA fragments that have been separated by gel electrophoresis. This stain binds specifically to DNA and fluoresces under UV light, allowing researchers to easily see and analyze DNA bands. Furthermore, it is more environmentally friendly compared to traditional stains, making it a safer option for laboratory settings. For instance, if you are running a gel to verify a PCR product, this stain will help you quickly assess the presence and size of your DNA fragments. -
Question:
How does SYBR Safe compare to other DNA stains?
Answer: SYBR Safe is ideally designed to be non-toxic and more biodegradable than other common DNA stains like ethidium bromide, which poses health risks. It effectively provides similar sensitivity and performance while being safer for researchers and the environment. When comparing the two, you will find that SYBR Safe allows for clear imaging with reduced exposure risks, making it perfect for high school experiments or professional labs where safety is a priority. -
Question:
Can SYBR Safe be used to stain RNA?
Answer: While EDVOTEK SYBR Safe DNA Stain is primarily designed for DNA visualization, it can also be used for RNA in specific contexts, although results may vary. The binding affinity is primarily for DNA, so if you're working with RNA samples, it might be more effective to use dedicated RNA stains. However, for mixed samples where you need to visualize any residual DNA alongside RNA, SYBR Safe could be integrated into your workflow with appropriate controls. -
Question:
What are the dilution guidelines for using SYBR Safe?
Answer: EDVOTEK SYBR Safe DNA Stain comes in a 10,000X concentration, meaning it should be diluted significantly before use. Generally, a dilution of 1:10,000 or 1:20,000 in your gel loading buffer is recommended for optimal results. Make sure to mix thoroughly before loading the samples into the gel. This specific dilution ensures that the dye provides clear fluorescence under UV light, allowing you to accurately visualize your DNA without overwhelming the signal. -
Question:
Is there a specific protocol for staining gels post-electrophoresis?
Answer: Yes, for optimal results with EDVOTEK SYBR Safe, post-electrophoresis staining is recommended. After you run your gel, immerse it in a diluted SYBR Safe solution for about 15-30 minutes. Then, rinse the gel to remove excess stain. This protocol ensures uniform staining of the DNA bands and enhances visibility when exposed to UV light, helping in detailed analysis of your samples. -
Question:
How should SYBR Safe be stored?
Answer: To maintain the quality of EDVOTEK SYBR Safe DNA Stain, it should be stored in a cool, dark place, preferably at -20°C. Protect it from light and repeated freeze-thaw cycles to ensure longevity and effectiveness. Proper storage is crucial since exposure to light can degrade the dye, affecting its performance in future experiments. This helps maintain consistent results across multiple uses in your laboratory. -
Question:
What safety precautions should be taken when using SYBR Safe?
Answer: Despite being safer than traditional DNA stains, it’s still important to wear gloves and goggles when handling SYBR Safe to minimize exposure to skin and eyes. Additionally, use in a well-ventilated area to ensure good air circulation. These precautions ensure safety in the lab environment, especially during activities such as loading gels or conducting post-staining analyses. Always follow lab safety protocols to maintain a secure working environment. -
Question:
Can I visualize DNA stained with SYBR Safe using visible light?
Answer: No, to visualize DNA stained with EDVOTEK SYBR Safe, you need a UV or blue light transilluminator. SYBR Safe fluoresces brightly under UV light, making the DNA bands visible against the gel background. Using visible light will not yield the same results, as the specific fluorescence characteristic is tailored for UV wavelengths. Thus, incorporating a suitable UV light source is essential for effective visualization. -
Question:
Where can I buy EDVOTEK SYBR Safe DNA Stain, 10,000X Concentrate, Green in Malawi?
Answer: You can purchase EDVOTEK SYBR Safe DNA Stain, 10,000X Concentrate, Green through Ubuy. They offer a reliable online platform where you can find a variety of laboratory supplies, including this stain. Ubuy provides a user-friendly shopping experience and delivery options that cater to customers in Malawi, making it easier for you to stock your lab with essential materials. -
Question:
What is the shelf life of SYBR Safe?
Answer: EDVOTEK SYBR Safe DNA Stain typically retains its effectiveness for about 12 months when stored properly at recommended temperatures. To ensure optimal performance, always check for any changes in color or consistency before use. Regularly monitoring the condition of your reagents guarantees that your experiments yield reliable results and helps maintain the integrity of your molecular biology work.
Edvotek Inc Science Kits & Toys Editorial Review
The SYBR Safe DNA Stain, 10,000 X is a highly recommended product for anyone working with DNA gels. Edvotek's SYBR Safe stain is the same product as Fisher Scientific's Invitrogen SYBR and is recommended for optimal results within 12 months of receipt. The product is easy to use, as 40 µl of the stain can be directly added from the tube to 400 mL of agarose gel. The stain can also be mixed with samples, used in a post-bath, or in-gel for staining. Detailed staining instructions can be found on Edvotek's website. The tube contains 80 µl of SYBR Safe DNA stain.
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Pros
- Easy to use
- Can be mixed with samples, used in post-bath, or in-gel
- Provides optimal results within 12 months of receipt
- Detailed staining instructions available on Edvotek's website
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MWK 260035
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Features & Benefits
- Safer than Ethidium Bromide
- More sensitive results
- 10,000 X Concentrate for 750 ml
- Volume: 80 µl
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