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Item #:
95488416
Bis-Tris (2-hydroxyethyl) Amino-tris(hydroxymethyl) Methane, 100G-5KG (500G) (100g)
Item #:
95488416
MWK 121653
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What Stands Out
High Purity
This Bis-Tris buffer offers exceptional purity, ensuring reliable and consistent results in biochemical applications, making it ideal for both research and industrial uses.
Versatile Concentrations
Available in multiple weights from 100g to 5kg, this product caters to diverse laboratory needs, allowing flexibility in experiment scaling and reducing waste.
Stable pH Buffer
Provides a stable pH range, crucial for maintaining enzyme activity and protein stability during experiments, thus delivering high-quality performance for sensitive applications.
Product Details
| Brand Name | Bioland |
| Manufacturer | Specialty Chemicals |
| Part Number | CB02 |
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Biochemists
Ideal for biochemists requiring a reliable buffer for protein and enzyme studies in research laboratories.
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Molecular Biologists
Beneficial for molecular biologists who need precise pH environments during DNA and RNA experiments.
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Educational Institutions
Recommended for educational settings needing quality reagents for teaching basic and advanced biochemical principles.
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Home Users
Not suitable for non-professionals lacking the knowledge or accessories to handle biochemical reagents safely.
Product Description
Bis-Tris (2-hydroxyethyl) Amino-tris(hydroxymethyl) Methane, 100G-5KG (500G) (100g)
Dietary Supplement Disclaimer
Statements regarding dietary supplements have not been evaluated by the Food and Drug Administration and are not intended to diagnose, treat, cure, or prevent any disease or health condition.
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Customer Questions & Answers
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Question:
What is Bis-Tris 2-hydroxyethyl Amino-trishydroxymethyl Methane used for?
Answer: Bis-Tris 2-hydroxyethyl Amino-trishydroxymethyl Methane, often referred to as Bis-Tris, is a buffering agent typically used in biochemical and molecular biology applications. It helps maintain a stable pH during biochemical reactions, crucial for enzyme activity and protein stability. For example, it is commonly used in SDS-PAGE electrophoresis and in buffer formulations for protein purification. -
Question:
What is the pH range of Bis-Tris buffers?
Answer: The pH range of Bis-Tris buffers typically lies between 6.1 and 7.5, making it suitable for a variety of biochemical experiments. This pH range is particularly effective for enzyme and protein applications, as many biological molecules function optimally within this window. It can be used to prepare buffers for applications like enzyme assays or various cell culture techniques. -
Question:
How should Bis-Tris be stored?
Answer: Bis-Tris should be stored in a cool, dry place away from direct sunlight and moisture. Ideally, it should be kept in a tightly sealed container to prevent contamination and degradation. Proper storage extends the product's shelf life, ensuring its effectiveness for laboratory use. This compound remains stable and functional when stored correctly, making it reliable for repeat experiments. -
Question:
Can Bis-Tris be used with other buffers?
Answer: Yes, Bis-Tris can be combined with other buffering agents, depending on the specific requirements of your assay. However, it is essential to consider the compatibility of the buffers, as mixing can sometimes alter the pH or ionic strength unexpectedly. You can use Bis-Tris along with other buffers in preparative methods where specific buffer properties are required, such as during protein purification procedures. -
Question:
Is Bis-Tris suitable for protein electrophoresis?
Answer: Absolutely! Bis-Tris is widely used for protein electrophoresis, especially in SDS-PAGE techniques. Its buffering capacity provides stable pH conditions that enhance the separation and resolution of proteins during electrophoresis. This is particularly important when analyzing protein samples, allowing for more precise comparisons in research and development settings. -
Question:
What is the solubility of Bis-Tris in water?
Answer: Bis-Tris is highly soluble in water, making it an ideal choice for preparing buffer solutions. This high solubility allows for easy preparation of concentrated solutions that can be diluted to desired concentrations based on experimental needs. For example, it can be easily dissolved in distilled water for preparing buffer stocks used in various laboratory applications. -
Question:
How do I prepare a Bis-Tris buffer solution?
Answer: To prepare a Bis-Tris buffer solution, dissolve the appropriate amount of Bis-Tris powder in distilled water and adjust the pH using hydrochloric acid or sodium hydroxide until the desired pH level is reached. Subsequently, dilute this solution to the desired final volume. This process allows scientists to create customized buffer solutions that meet specific experimental requirements. -
Question:
What concentrations of Bis-Tris are commonly used?
Answer: Common concentrations for Bis-Tris buffer solutions range from 20 mM to 200 mM, depending on the application. Lower concentrations are often sufficient for general applications, while higher concentrations may be utilized in scenarios requiring stricter pH control. This versatility allows researchers to adapt the buffer according to the sensitivity of their biochemical assays. -
Question:
What are the available sizes of Bis-Tris for purchase?
Answer: Bis-Tris is available in multiple sizes, typically ranging from 100 grams to 5 kilograms. This variety allows users to choose a quantity that best fits their laboratory needs. Smaller sizes are ideal for research laboratories with limited immediate needs, whereas larger sizes benefit high-throughput labs or facilities conducting extensive experiments. -
Question:
Where can I buy Bis-Tris 2-hydroxyethyl Amino-trishydroxymethyl Methane in Malawi?
Answer: You can purchase Bis-Tris 2-hydroxyethyl Amino-trishydroxymethyl Methane from Ubuy in Malawi. Ubuy offers a wide selection of laboratory chemicals, including Bis-Tris in various quantities, making it a convenient source for researchers and laboratories looking for reliable products for their experiments.
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